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. 2023 Feb 24;222(4):e202211031. doi: 10.1083/jcb.202211031

Figure 1.

Figure 1.

ADAM23 is required for Kv1 complexes to accumulate at the JXP of myelinated axons in both PNS and CNS. (A) Left: Immunohistochemistry (IHC) on adult WT mouse sciatic nerve axon immunolabeled with antibodies against CASPR (gray), Kv1.1 (green), and ADAM23 (red). Right: Line-scan quantification of fluorescence distribution in ADAM23 and Kv1 staining showing a close overlap in the JXP expression patterns of the two proteins. Scale bar = 10 µm. (B) Sciatic nerve IHC on adult mouse teased sciatic nerve axon immunolabeled with CASPR (gray) and ADAM23 (red). Scale bar = 10 µm. Middle and right panels are magnified inverted grayscale images of CASPR and ADAM23 expression in the mesaxonal line region (stippled box in first panel) as indicated by the red arrowheads. Scale bar = 2 µm. (C and D) IHC on P10 WT (top) and Adam23Δ1/Δ1 (A23KO, bottom) mouse teased sciatic nerve (C) and longitudinal 12 μm spinal cord sections from the cervical region (D). All images contain immunolabeling for CASPR (gray) and either ADAM23, Kv1.1, Kv1.2, CASPR2, or βIV Spectrin (red). Scale bar = 10 μm (applies to all images in C and D).