Figure 5.
Axonal expression levels of Kv1 complex proteins in the absence of ADAM23. (A) Representative Western blot results from homogenized sciatic nerves of P10 WT (Adam23+/+) and Adam23Δ1/Δ1 mice. Each lane represents a pair of sciatic nerves from one mouse. All experiments were performed in triplicate (three biological replicates per genotype per blot). Staining includes ADAM23, CASPR2, Kv1.1, Kv1.2, PSD95, TAG-1, P0 (MPZ), and Neurofilament (NFM). (B) Band density for each protein was normalized against P0 and graphed depending on genotype. Each dot represents one mouse, i.e., one pair of sciatic nerves. ADAM23 n = 9, Kv1.1 n = 6, Kv1.2 n = 9, CASPR2 n = 9, PSD95 n = 9 and TAG-1 n = 3. Unpaired t test was carried out to determine statistical significance of the results. ADAM23, F (8, 8) = 7.0, ****P < 0.0001; Kv1.1, F (5, 5) = 2.6, P = 0.37; Kv1.2, F (8, 8) = 1.2, P = 0.47; CASPR2, F (8, 8) = 2.3, P = 0.41; PSD95, F (8, 8) = 1.2, P = 0.98; TAG-1, F (8, 8) = 1.6, P = 0.33. Source data are available for this figure: SourceData F5.