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. 2023 Feb 24;222(4):e202211031. doi: 10.1083/jcb.202211031

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© 2023 Kozar-Gillan et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 8. — JXP Kv1 channels do not affect axonal excitability or amplitude but regulate the length of refractory period. (A) CAP recording set-up, with stimulating and recording glass suction electrodes attached to either end of the L4/L5 dorsal root. (B) Representative population CAP recording at 100 μA showing the fast (Aαβ), medium (Aδ), and slow (C) conducting components (arrow), each displaying a characteristic triphasic (positive-negative-positive) response. (C) No effect of genotype on Aαβ-fiber activation thresholds (unpaired t test, P = 0.44). Sample size: wild-type (WT) dorsal root n = 9 (three mice); Adam23^PvKO/PvKO dorsal root n = 11 (three mice). (D) Stimulus-response curve showing increasing Aαβ amplitude with increasing stimulus intensity with no significant effect of genotype on Aαβ amplitude (two-way ANOVA genotype, F(1,342) = 1.02, P = 0.31; intensity, F(18,342) = 6.09, ****P < 0.0001). Sample size: wild-type (WT) dorsal root n = 9 (three mice); Adam23^PvKO/PvKO dorsal root n = 11 (three mice). (E) Average estimated conduction velocity was not altered by genotype or 4-AP treatment (RM two-way ANOVA, genotype, F(1,18) = 1.85, P = 0.19; 4-AP, F(1,18) = 0.02, P = 0.88). Sample size: wild-type (WT) dorsal root n = 9 (three mice); Adam23^PvKO/PvKO dorsal root n = 11 (three mice). (F) Representative Aαβ traces at a 10-ms inter-pulse interval (IPI) illustrating the amplitudes of the 1st and 2nd Aαβ CAP in dorsal roots from WT mice (Fi), WT mice + 4-AP(Fii), Adam23^PvKO/PvKO mice (Fiii) and Adam23^PvKO/PvKO mice + 4-AP(Fiv). Double-headed arrow illustrates the Aαβ-fiber amplitude. (G) Decreasing IPI decreased the 2^nd CAP/1^st CAP ratio in both WT (n = 9 dorsal roots/3 mice) and Adam23^PvKO/PvKO (n = 11 dorsal roots/3 mice) nerves in a genotype-dependent manner (RM two-way ANOVA: genotype, F(1,170) = 6.54, *P = 0.01; IPI, F(9,170) = 32.39, ****P < 0.0001), revealing a significant increased refractory period in Aαβ fibers of Adam23^PvKO/PvKO mice (H) 4-AP decreased the 2nd CAP/1st CAP ratio in WT mice (RM two-way ANOVA: 4-AP, F(1,134) = 45.28, ****P < 0.0001, IPI, F(9,134) = 47.17, ****P < 0.0001). WT (n = 8 dorsal roots/3 mice) vs. WT + 4-AP (n = 8 dorsal roots/3 mice). (I) 4-AP did not alter the 2^nd CAP/1^st CAP ratio in Adam23^PvKO/PvKO mice (RM Two-way ANOVA: 4-AP, F(1,127) = 0.77, P = 0.38; IPI, F(9,127) = 18.55, ****P < 0.0001). Adam23^PvKO/PvKO (n = 8 dorsal roots/3 mice) vs. Adam23^PvKO/PvKO + 4-AP (n = 8 dorsal roots/3 mice).