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. 2023 Feb 24;220(5):e20221346. doi: 10.1084/jem.20221346

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© 2023 Nickerson et al.

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Figure 5. — CD11c⁺CD11b⁺ ABCs self-renew or differentiate. Sorted ABCs (CD19⁺ CD11c⁺ CD11b⁺ CD138⁻) or CD44^low B cells (CD19⁺ CD44^low CD138⁻ CD11c⁻ CD11b⁻) isolated from spleens of CD45.1⁺ MRL/lpr females at 14–20 wk of age were VPD labeled and adoptively transferred into preautoimmune female CD45.2 MRL/lpr recipients. Recipient spleens were analyzed at d7, d14, or d21 following transfer. (A) Representative staining of total live splenocytes at d21 after transfer from mice receiving ABCs (left), CD44^low B cells (middle), or no transfer controls (right). (B) Frequency of transferred CD45.1⁺ ABCs (red) or CD44^low B cells (black) in recipient spleens at indicated times after transfer. (C) VPD staining from mice in A gated on CD45.1⁺ transferred cells. (D) Proportion of VPD undiluted cells among transferred cells. (E) Proportion of CD11c⁺CD11b⁺ ABCs among transferred cells. (F) Proportion of CD44⁺CD138⁺ PBs among transferred cells. (G) Proportion of PNA⁺CD38^low GC B cells among transferred cells. Data are pooled from five independent sorts/transfers with 3–4 experiments per timepoint. Statistical comparisons by two-tailed Mann-Whitney; *P < 0.05; **P < 0.01; ***P < 0.001.