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. 2023 Feb 24;220(5):e20221346. doi: 10.1084/jem.20221346

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© 2023 Nickerson et al.

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Figure S3. — Isolation and characterization of CD19⁺ MRL/lpr B cells by scRNA-seq. (A) CD19⁺ TCRβ⁻ sort gates are indicated. Sorted B cells were stained with oligo-tagged sample hashing and surface feature antibodies prior to being pooled and processed via the 10× Chromium Next GEM Single Cell V(D)J v1.1 protocol per the manufacturer’s instructions. (B) Purple dots indicate contribution of cells from each donor mouse to the overall clustering UMAP. (C) A separate aliquot of total splenocytes from the same mice in A were stained with the indicated markers for conventional flow cytometry. (D) Summary of the contribution of each mouse to the clusters in Fig. 7 A as determined by the sample-hashing oligo-tagged anti-CD45/anti-β2m antibodies included in the surface feature library. (E) Expression of Itgam (CD11b), Itgax (CD11c), Spn (CD43), and Cd5 genes overlaid on the cluster UMAP. (F) Expression of CITE-seq mapped surface markers in scRNA-seq. Pairwise plots of normalized counts from oligo-tagged surface feature antibodies for B cell clusters not included in Fig. 7 C.