TABLE 2.
Quantitative data of PVN, NTS, and RVLM capillaries’ ultrastructure in the 4 experimental groups.
| Wistar-S | Wistar –T | SHR-S | SHR-T | |
|---|---|---|---|---|
| PVN | ||||
| Luminal perimeter (µm) | 17.44 ± 0.55 | 18.98 ± 0.87 | 22.81 ± 0.75* | 24.46 ± 1.20* |
| Abluminal perimeter (µm) | 19.28 ± 0.58 | 21.49 ± 0.98† | 25.97 ± 0.87* | 27.28 ± 1.23 |
| Lumen diameter (µm) | 5.55 ± 0.18 | 6.04 ± 0.28 | 7.26 ± 0.24* | 7.79 ± 0.38* |
| Endothelial cell area (µm2) | 5.63 ± 0.48 | 7.22 ± 0.61 | 12.04 ± 1.26* | 9.55 ± 0.80 |
| Basement membrane thickness (µm) | 0.072 ± 0.002 | 0.080 ± 0.003 | 0.075 ± 0.004 | 0.064 ± 0.003* |
| Transcytotic vesicles/capillary (n) | 4.69 ± 0.43 | 3.00 ± 0.46 | 8.03 ± 1.00* | 3.61 ± 0.60† |
| Capillary border extension (µm) | 1.39 ± 0.17 | 0.81 ± 0.09† | 1.25 ± 0.13 | 1.09 ± 0.12 |
| Tight junction extension (µm) | 0.54 ± 0.08 | 0.56 ± 0.06 | 0.60 ± 0.07 | 0.74 ± 0.10 |
| Tight junction/capillary border (%) | 40 ± 4 | 72 ± 4† | 47 ± 4 | 67 ± 6† |
| Pericytes’ coverage (%) | 31 ± 2 | 43 ± 3† | 29 ± 3 | 29 ± 3* |
| NTS | ||||
| Luminal perimeter (µm) | 17.17 ± 0.49 | 18.51 ± 0.67 | 19.73 ± 0.85* | 17.27 ± 0.53 |
| Abluminal perimeter (µm) | 19.49 ± 0.52 | 21.43 ± 0.75 | 22.77 ± 0.99* | 19.42 ± 0.61† |
| Lumen diameter (µm) | 5.46 ± 0.16 | 5.89 ± 0.22 | 6.28 ± 0.27* | 5.50 ± 0.17 |
| Endothelial cell area (µm2) | 7.02 ± 0.65 | 9.15 ± 0.86 | 10.18 ± 1.09* | 6.24 ± 0.71† |
| Basement membrane thickness (µm) | 0.068 ± 0.003 | 0.090 ± 0.004† | 0.061 ± 0.002 | 0.073 ± 0.006* |
| Transcytotic vesicles/capillary (n) | 4.93 ± 0.63 | 3.96 ± 0.57 | 7.29 ± 0.98 | 4.13 ± 0.60† |
| Capillary border extension (µm) | 0.87 ± 0.10 | 1.13 ± 0.12 | 1.32 ± 0.16 | 1.06 ± 0.12 |
| Tight junction extension (µm) | 0.43 ± 0.06 | 0.71 ± 0.06† | 0.53 ± 0.10 | 0.68 ± 0.08 |
| Tight junction/capillary border (%) | 46 ± 5 | 66 ± 4† | 39 ± 4 | 65 ± 4† |
| Pericytes’ coverage (%) | 24 ± 2 | 40 ± 4† | 26 ± 3 | 25 ± 3* |
| RVLM | ||||
| Luminal perimeter (µm) | 15.69 ± 0.43 | 16.04 ± 0.44 | 18.29 ± 0.66* | 16.95 ± 0.73 |
| Abluminal perimeter (µm) | 17.75 ± 0.49 | 19.02 ± 0.50 | 20.27 ± 0.71* | 18.64 ± 0.78 |
| Lumen diameter (µm) | 4.99 ± 0.14 | 5.11 ± 0.14 | 5.82 ± 0.21* | 5.40 ± 0.23 |
| Endothelial cell area (µm2) | 5.55 ± 0.60 | 8.16 ± 0.84† | 6.28 ± 0.59 | 5.35 ± 0.46* |
| Basement membrane thickness (µm) | 0.072 ± 0.002 | 0.078 ± 0.002 | 0.058 ± 0.002* | 0.063 ± 0.003* |
| Transcytotic vesicles/capillary (n) | 4.53 ± 0.59 | 4.13 ± 0.50 | 7.44 ± 0.85* | 3.96 ± 0.77† |
| Capillary border extension (µm) | 1.28 ± 0.16 | 1.11 ± 0.11 | 1.22 ± 0.22 | 1.30 ± 0.26 |
| Tight junction extension (µm) | 0.71 ± 0.11 | 0.77 ± 0.09 | 0.78 ± 0.17 | 0.90 ± 0.16 |
| Tight junction/capillary border (%) | 55 ± 4 | 71 ± 5 | 60 ± 5 | 71 ± 5 |
| Pericytes’ coverage (%) | 33 ± 2 | 26 ± 2 | 28 ± 3 | 34 ± 3 |
Values are means ± SEM. n = 25–30 capillaries/group, 3 rats/group. Comparisons made by Factorial Two-way ANOVA. PVN Luminal perimeter: group F (1,108) = 41.08, p < 0.001, condition F (1,108) = 3.55, p = 0.062, group x condition F (1,108) = 0.01, p = 0.948; Abluminal perimeter: group F (1,108) = 26.61, p < 0.001, condition F (1,108) = 12.59, p = 0.001, group x condition F (1,108) = 4.50, p = 0.036; Lumen diameter: group F (1,108) = 40.78, p < 0.001, condition F (1,108) = 3.54, p = 0.062, group x condition F (1,108) = 0.01, p = 0.948; Endothelial cell area: group F (1,108) = 26.67, p < 0.001, condition F (1,108) = 0.28, p = 0.596, group x condition F (1,108) = 5.81, p = 0.018; Basement membrane thickness: group F (1,108) = 4.44, p = 0.038, condition F (1,108) = 0.24, p = 0.628, group x condition F (1,108) = 9.47, p = 0.003; Transcytotic vesicles/capillary: group F(1,108) = 8.70, p = 0.004, condition F(1,108) = 20.83, p < 0.001, group x condition F(1,108) = 4.16, p = 0.044; Capillary border extension: group F (1,88) = 0.25, p = 0.618, condition F (1,88) = 7.02, p = 0.010, group x condition F (1,88) = 2.26, p = 0.136; Tight junction extension: group F (1,88) = 2.28, p = 0.134, condition F (1,88) = 1.02, p = 0.317, group x condition F (1,88) = 0.57, p = 0.452; Tight junction/capillary border: group F (1,88) = 0.02, p = 0.877, condition F (1,88) = 31.96, p < 0.001, group x condition F (1,88) = 1.61, p = 0.208; Pericytes coverage: group F (1,102) = 9.81, p = 0.002, condition F (1,102) = 4.61, p = 0.034, group x condition F (1,102) = 4.30, p = 0.041; NTS: Luminal perimeter: group F (1,116) = 1.03, p = 0.312, condition F (1,116) = 0.74, p = 0.391, group x condition F (1,116) = 8.53, p = 0.004; Abluminal perimeter: group F (1,116) = 0.74, p = 0.392, condition F (1,116) = 0.91, p = 0.342, group x condition F (1,116) = 12.81, p < 0.001; Lumen diameter: group F (1,116) = 1.08, p = 0.301, condition F (1,116) = 0.71, p = 0.400, group x condition F (1,116) = 8.54, p = 0.004; Endothelial cell area: group F (1,116) = 0.02, p = 0.883, condition F (1,116) = 1.15, p = 0.286, group x condition F (1,116) = 12.91, p < 0.001; Basement membrane thickness: group F (1,116) = 8.86, p = 0.004, condition F (1,116) = 17.78, p < 0.001, group x condition F (1,116) = 1.54, p = 0.217; Transcytotic vesicles/capillary: group F (1,116) = 3.13, p = 0.079, condition F (1,116) = 8.35, p = 0.005, group x condition F (1,116) = 2.35, p = 0.128; Capillary border extension: group F (1,106) = 2.27, p = 0.135, condition F (1,106) = 0.00, p > 0.999, group x condition F (1,106) = 4.25, p = 0.042; Tight junction extension: group F (1,106) = 0.42, p = 0.839, condition F (1,106) = 10.20, p = 0.002, group x condition F (1,106) = 0.37, p = 0.542; Tight junction/capillary border: group F (1,106) = 1.42, p = 0.237, condition F (1,106) = 31.43, p < 0.001, group x condition F (1,106) = 0.71, p = 0.401; Pericytes coverage: group F (1,112) = 4.40, p = 0.038, condition F (1,112) = 5.78, p = 0.018, group x condition F (1,112) = 6.73, p = 0.011; RVLM Luminal perimeter: group F (1,110) = 9.32, p = 0.003, condition F (1,110) = 0.74, p = 0.391, group x condition F (1,110) = 2.16, p = 0.145; Abluminal perimeter: group F (1,110) = 2.90, p = 0.091, condition F (1,110) = 0.08, p = 0.775, group x condition F (1,110) = 5.33, p = 0.023; Lumen diameter: group F (1,110) = 9.37, p = 0.003, condition F (1,110) = 0.67, p = 0.414, group x condition F (1,110) = 2.18, p = 0.143; Endothelial cell area: group F (1,110) = 2.65, p = 0.106, condition F (1,110) = 1.73, p = 0.191, group x condition F (1,110) = 7.67, p = 0.007; Basement membrane thickness: group F (1,110) = 40.95, p < 0.001, condition F (1,110) = 5.89, p = 0.017, group x condition F (1,110) = 0.05, p = 0.826; Transcytotic vesicles/capillary: group F (1,110) = 3.93, p = 0.049, condition F (1,110) = 7.88, p = 0.006, group x condition F (1,110) = 4.97, p = 0.028; Capillary border extension: group F (1,89) = 0.11, p = 0.742, condition F (1,89) = 0.05, p = 0.820, group x condition F (1,89) = 0.40, p = 0.527; Tight junction extension: group F (1,89) = 0.48, p = 0.488, condition F (1,89) = 0.39, p = 0.5533, group x condition F (1,89) = 0.04, p = 0.835; Tight junction/capillary border: group F (1,89) = 0.31, p = 0.578, condition F (1,89) = 7.90, p = 0.006, group x condition F (1,89) = 0.19, p = 0.662; Pericytes coverage: group F (1,110) = 0.27, p = 0.607, condition F (1,110) = 0.01, p = 0.918, group x condition F (1,110) = 7.05, p = 0.009. Significances (p < 0,05) * vs. respective Wistar, † vs. respective S.