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. 2023 Mar 9;12:e70792. doi: 10.7554/eLife.70792

Figure 2. Neuropathological changes in the subcortical white matter in forebrain proteolipid protein (PLP) conditional knockout (cKO) mice.

(A) Immunofluorescent characterization of microgliosis (IBA1 immunolabeling) in forebrain PLP cKO mice (overview coronal brain section). Quantifications for IBA1+area for region of interests (ROIs) (CTX: cortex, CC: corpus callosum, STR: striatum) are shown on the right. Bars represent means ± SEM and datapoints represent individual mice (n=4 per group). For statistical analyses two-sided Student’s t-tests were performed. Age of the animals was 22 months. (B) Immunofluorescent characterization of astrogliosis (GFAP immunolabeling) in forebrain PLP cKO mice (overview coronal brain section). Quantifications for GFAP+area for ROIs (CTX: cortex, CC: corpus callosum, STR: striatum) are shown on the right. Bars represent means ± SEM and datapoints represent individual mice (n=4 per group). For statistical analyses two-sided Student’s t-tests were performed. Age of the animals was 22 months. (C) Immunohistochemical characterization of axonal swellings (amyloid precursor protein [APP] immunolabeling) in forebrain PLP cKO mice (overview coronal brain section). Note the apparent fimbria atrophy and ventricular enlargement in forebrain PLP cKO mice. Age of analysis 22 months. (C’) Closeup images of boxed areas in C. Black arrows indicate axonal spheroids in the fimbria and internal capsule of cKO mice, which are essentially absent in Ctrl mice. Levels of significance were defined as p>0.05 (*), p>0.01 (**) and p>0.001 (***).

Figure 2.

Figure 2—figure supplement 1. Neuropathological changes in the subcortical white matter in forebrain proteolipid protein (PLP) conditional knockout (cKO) mice.

Figure 2—figure supplement 1.

(A,E,I) Representative light microscopic images of cross-sectioned fimbria, corpus callosum, and prefrontal cortex immunolabeled for amyloid precursor protein (APP) in forebrain PLP cKO and control mice at age 22 months. (B,F,J) Representative light microscopic images of cross-sectioned fimbria, corpus callosum, and prefrontal cortex immunolabeled for MAC3 in forebrain PLP cKO and control mice at age 22 months. (C,G,K) Representative light microscopic images of cross-sectioned fimbria, corpus callosum, and prefrontal cortex immunolabeled for IBA1 in forebrain PLP cKO and control mice at age 22 months. (D,H,L) Representative light microscopic images of cross-sectioned fimbria, corpus callosum, and prefrontal cortex immunolabeled for GFAP in forebrain PLP cKO and control mice. Analyses were performed at 22 months. Genotype-dependent quantifications are shown on the right. Bars represent means ± SEM; datapoints represent individual animals; male and female mice are indicated by squares and triangular datapoints, respectively. Scale bars 50 µm. For statistical analyses two-sided Student’s t-tests were performed for the Ctrl versus cKO comparison. p-Values are given in the bar graphs. All data are shown as means ± SEM. Levels of significance were defined as p>0.05 (*), p>0.01 (**) and p>0.001 (***).