Figure 3. Behavioral deep-phenotyping of male and female forebrain proteolipid protein (PLP) conditional knockout (cKO) mice.

Heatmap representation of behavioral data in the categories of overall health status, motor function and coordination, hearing, vision, heat/pain perception, sensorimotor gating, social behavior, depression-like behavior, cognitive performance in the IntelliCage, spatial memory and reversal in the Morris water maze (MWM), exploration and working memory, catatonic signs and executive function. Each heatmap column represents individual animals (n-numbers). Statistical significance is indicated by red dots. Raw values, n-numbers of mice tested per experiment, mean, SEM, and p-values are specified in Table 1.

Figure 3—figure supplement 1. Forebrain proteolipid protein (PLP) conditional knockout (cKO) mice display normal latency to fall from the rotarod.

(A) Representative image of a mouse subjected to the rotarod test assessing motor performance. (B) Groups of female and male PLP cKO (n=19 or 14, respectively) and Ctrl (n=16 per sex) mice were assessed in the rotarod at ages 6 months on 2 consecutive days (day 1 and day 2). (C) Groups of female and male PLP cKO (n=18 or 14, respectively) and Ctrl (n=16 per sex) mice were assessed in the rotarod at ages 17 months on 2 consecutive days (day 1 and day 2). Note that cKO mice performed normally on the rotarod. Bars represent means ± SEM. Datapoints represent individual mice; not significant (n.s.) by repeated measures two-way analysis of variance (ANOVA). Levels of significance were defined as p>0.05 (*), p>0.01 (**) and p>0.001 (***).