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. 2023 Mar 9;12:e84740. doi: 10.7554/eLife.84740

Figure 1. Suppression of REM sleep following CNO injection.

(a) Time course of wakefulness, NREM, and REM sleep in the 6 hr following intraperitoneal injections of CNO or saline at light onset (ZT 0). (b) Percentage of time spent in the three vigilance states during the first 2 hr (left column) and over the entire 6 hr observation period (right column) after saline and CNO injections. Note that REM sleep is presented both as proportion of the recording time (third row) and of the total sleep time (fourth row). n=16 for saline, n=11 for 1 mg/kg, n=15 for 5 mg/kg, n=14 for 10 mg/kg. Asterisks indicate post hoc comparisons with significant differences (*p<0.05, **p<0.01, ***p<0.001) for analyses with significant main effects. CNO: clozapine-N-oxide. NREM: non-rapid eye movement sleep. REM: rapid eye movement sleep. TST: total sleep time. ZT: zeitgeber time.

Figure 1.

Figure 1—figure supplement 1. Reduced REM/NREM ratio following CNO injections.

Figure 1—figure supplement 1.

(a) Time course of REM sleep expressed as ratio between REM and NREM sleep over the 6 hr following intraperitoneal injections of CNO or saline at light onset (ZT 0). (b) REM/NREM ratio during the first 2 hr (left column) and over the entire 6 hr observation period (right column) after saline and CNO injections. n=16 for saline, n=11 for 1 mg/kg, n=15 for 5 mg/kg, n=14 for 10 mg/kg for vigilance state analysis. Asterisks indicate post hoc comparisons with significant differences (*p<0.05, **p<0.01, ***p<0.001) for analyses with significant main effects. CNO: clozapine-N-oxide. EEG: electroencephalogram. NREM: non-rapid eye movement sleep. REM: rapid eye movement sleep. ZT: zeitgeber time.