Figure 4. Orai1 is dispensable for B cell development.

(A–B) Flow cytometric analysis of bone marrow populations for B cell fractions A (B220⁺CD43⁺HSA⁻BP-1⁻), B (B220⁺CD43⁺HSA⁺BP-1⁻), C (B220⁺CD43⁺HSA⁺BP-1⁺), D (B220⁺CD43⁻IgM⁻CD93⁺), E (B220⁺CD43⁻IgM⁺CD93⁺), and F (B220⁺CD43⁻IgM⁺CD93⁻). (C) Quantification of bone marrow populations in (A, B) (n=7 and six biological replicates; Mann-Whitney test). (D) Flow cytometric analysis of isolated populations in the spleen for B cell developmental stages T1 (B220⁺AA4.1⁺CD23⁻IgM⁺), T2 (B220⁺AA4.1⁺CD23⁺IgM⁺), and T3 (B220⁺AA4.1⁺CD23⁺IgM⁻). (E) Flow cytometric analysis of isolated populations in the spleen for marginal zone (MZ) B cells (B220⁺CD93⁻CD23⁻IgM⁺) and follicular (FO) B cells (B220⁺CD93⁻CD23⁺IgM⁺). (F) Quantification of splenic populations in (D, E) (n=7 and six biological replicates; Mann-Whitney test). All scatter plots are presented as mean ± SEM.