FIG. 11.

Analysis of macrophage and mouse infections by L. mexicana wild type and mutants. (A, D) Infection of peritoneal macrophages by L. mexicana wild type, ΔPMM, ΔPMM + pXPMM, ΔPMM + cRIBPMM (A) or by L. mexicana wild type, ΔDPMS, ΔDPMS + pXDPMS, ΔDPMS + cRIBDPMS (D). Peritoneal macrophages were infected at a ratio of two stationary phase promastigotes per cell. The percentage of infected macrophages (sample size, 300) was counted 6 days after the infection. The standard errors of duplicate experiments are indicated. (B, C, E) For in vivo infection experiments, BALB/c mice were challenged with 10⁷ L. mexicana promastigotes in the right hind footpad. The swelling caused by L. mexicana wild type, ΔPMM, and ΔPMM + pXPMM (B), by L. mexicana wild type, ΔPMM, and ΔPMM + cRIBPMM (C), and by L. mexicana wild type, ΔDPMS, ΔDPMS + pXDPMS, or ΔDPMS + pRIBDPMS (E) was recorded. The infection experiments were performed in quadruplicates and the standard error is indicated.