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Targeted gene replacement and gene addback of the PMM and DPMS alleles. (A) Restriction maps of the PMM and DPMS loci. The resistance genes BLE and HYG and the primer binding sites (KO1 to KO4) used for the construction of gene deletion cassettes are indicated. (B) Restriction map of the chromosomal gene addback cassette for genetic rescue of the L. mexicana ΔPMM and ΔDPMS mutants.
