FIG. 6.
Enzyme activities, growth curve, and Man content of L. mexicana wild-type (WT) and mutant promastigotes. (A) Enzymatic activity of phosphomannomutase (PMM), phosphomannose isomerase (PMI), and hexokinase (HK) in freeze/thaw/sonication lysates of wild-type L. mexicana and a ΔPMM mutant. (B) Synthesis of lipid-bound [14C]Man by microsomal fractions of L. mexicana wild-type, ΔDPMS, and ΔDPMS + cRIBDPMS promastigotes. The bars represent the average of duplicate assays. (C) Growth curves of L. mexicana wild-type and ΔPMM mutant promastigotes with and without Man supplementation of the medium. (D) Man content of membranes from L. mexicana wild-type and several mutant promastigotes, as determined by gas chromatography-mass spectrometry.