FIG. 5.

E-cadherin expression is retained in the mesoderm of Sna^−/− mutant embryos. (A) Whole-mount in situ hybridization with E-cadherin antisense riboprobes of a control littermate (left) and a Sna^−/− embryo (right). (B and C) Plastic sections of embryos treated as described for panel A. E-cadherin RNA expression is downregulated in the mesoderm of the control littermate embryo (B), but expression is retained (arrows) in the mesoderm of the Sna^−/− embryo (C). (D to G) Immunofluorescence with anti-E-cadherin monoclonal antibody. (D and E) Nomarski optics. (F and G) Fluorescence optics. In the Sna^−/− embryo (G), E-cadherin protein expression is retained in the mesoderm layer (arrows). Abbreviations: ee, embryonic ectoderm; m, mesoderm; ps, primitive streak.