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. 2023 Jan 26;7(3):414–423. doi: 10.1038/s41559-022-01975-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — Venn diagrams outlining the numbers of significantly differentially expressed genes for a given comparison (separated by a ‘v’). T1/S1/T2/S2 correspond to populations, (C) corresponds to control conditions, (Z) to zinc conditions: for example, T1(C) v S1(C) refers to genes differentially expressed between T1 in the control, and S1 in the control. A ‘+’ corresponds to genes that fulfil both sets of criteria: for example, T1 (C) v T1 (Z) + T2 (C) v T2 (Z) corresponds to the set of genes that are differentially expressed between both (i) T1 in the control vs. T1 in the zinc, and (ii) T2 in the control vs. T2 in the zinc. Panels illustrate (A) between treatment expression changes within populations, and their overlaps, (B) between ecotype-changes in the zinc in the two geographic groups, and their overlap, (C) the overlap between genes differentially expressed in both tolerant populations between conditions, both susceptible populations between conditions, and between both ecotype pairs within the zinc treatment, and (D) between-ecotype changes in the control in the two geographic groups, and their overlap.