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mCherry intensity on microtubules in the kinesin pretreatment assay.
| KIF5C concentration | mCherry intensity (a. u., mean ± S.D.) | GMPCPP/GDP (mean ± S.E.) | |
|---|---|---|---|
| GDP-MT | GMPCPP-MT | ||
| 0 µM | 26 ± 15 (n = 75) | 94 ± 17 (n = 75) | 3.61 ± 0.18 |
| 0.1 µM | 31 ± 11 (n = 75) | 93 ± 12 (n = 75) | 3.02 ± 0.15 |
| 0.5 µM | 50 ± 12 (n = 75) | 117 ± 17 (n = 75) | 2.34 ± 0.14 |
| 2 µM | 91 ± 22 (n = 75) | 170 ± 32 (n = 75) | 1.86 ± 0.04 |
| 2 μM (ADP wash) | 32 ± 13 (n = 75) | 116 ± 18 (n = 75) | 3.69 ± 0.21 |
