Skip to main content
. Author manuscript; available in PMC: 2024 Apr 1.
Published in final edited form as: Gut. 2022 Oct 3;72(4):654–662. doi: 10.1136/gutjnl-2021-326305

Figure 1. Stem cell expressed NOX1 is required for TNFα induced ROS production and NOX1 deficiency results in secretory precursor enhancement.

Figure 1

A. TNFα (left) and RANKL (right) induced ROS levels in Nox1WT and Nox1KO cells (25% WRN, 20 μM DAPT, measured 3 days after plating). Ns, non-significant. **, P < 0.01. ***, P < 0.001. (Mann-Whitney test, n = 6 to 8 per group). B, C, D. Cell type specific gene expression changes with decreasing Notch (left) and Wnt (right) culture conditions stratified by Nox1 genotype. RT-PCR results for Muc2 (B--goblet cells), Reg4 (C--deep crypt secretory cells) and Car1 (D--colonocytes) for Nox1WT (black) and Nox1KO (gray) colonoids normalized to a house keeping gene, Hprt1 (n=4 per group). *, P < 0.05 (Mann-Whitney test) E. Heatmap of average NOX1 and DUOX2 expression from uninflamed biopsy human (scRNAseq) of UC. F. NOX1 and DUOX2 expression from uninflamed human colonoid lines bulk RNA seq data. human colonoid lines (n = 7 lines) were cultured under 50%WRN (stem cells are the major cell type) or 5% WRN (colonocytes are major cell type). **, P < 0.01 (Mann-Whitney test).