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. Author manuscript; available in PMC: 2024 Apr 1.
Published in final edited form as: Gut. 2022 Oct 3;72(4):654–662. doi: 10.1136/gutjnl-2021-326305

Figure 4. scRNASeq identifies marked M cell induction with Nox1-deficiency plus TNFα stimulation, rescued by H2O2 and paraquat.

Figure 4

A. UMAP of joint subclustering (left) of putative colonoid DCS/goblet cells clustering. Heatmap (Right) of top 10 upregulated genes in each cluster from sub-clustering of putative DCS/goblet cells from colonoid scRNAseq shows unexpected presence of M cells. B. Stacked bar graph of subcluster percentages stratified by Nox1 genotype and TNFα treatment. C. Heatmap of known M cell markers of putative colonoid DCS/goblet cells. D. UEA1 staining of Nox1WT and deficient monolayer cells stimulated with TNFα or RANKL for 72 hours. Representative images of each group are shown. Scale bar denotes 200μm. Quantification of M cell percentage was performed on 42 images for each treatment group. *, P < 0.05 (two-sided Wilcoxon signed-rank test). E. Representative UEA1 staining of TNFα or RANKL stimulated NOX1KO colonoids co-treated with H2O2 or Paraquat. M cell percentages were quantitated on 42 images per treatment group. *, P <0.05 (two-sided Wilcoxon signed-rank test).