KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| NRF2 | Abcam | Cat#: ab62352 |
| KEAP1 | Cell Signaling Tech | Cat#: 7705 |
| HMOX1 | Proteintech | Cat#: 10701–1-AP |
| NQO-1 | Cell Signaling Tech | Cat#: 3187S |
| HK2 | Cell Signaling Tech | Cat#: 2867T |
| ALDH3A1 | Novus Biologicals | Cat#: NBP2–15340 |
| FLAG | Sigma | Cat#: F7425 |
| β-ACTIN | Cell Signaling Tech | Cat#: 4970 |
| HRP-labeled anti-mouse | Cell Signaling Tech | Cat#: 7076 |
| HRP-labeled anti-rabbit | Cell Signaling Tech | Cat#: 7074 |
| Bacterial and Virus Strains | ||
| DH5α | Fisher Scientific | Cat#:18265017 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| DMEM | Corning | Cat#:15–013-CV |
| RPMI-1640 | Corning | Cat#: ICN1646454 |
| FBS | Corning | Cat#: 35–010-CV |
| GlutaMax | Invitrogen | Cat#: 35050061 |
| Penicillin-Streptomycin | Millipore | Cat#: P0781 |
| Blasticidin | Fisher Scientific | Cat#: 50712728 |
| Puromycin | Sigma-Aldrich | Cat#: P8833 |
| Sequencing grade modified trypsin | Promega | Cat#: V5111 |
| DMSO | Fisher Scientific | Cat#: MT-25950CQC |
| Urea | VWR Intl | Cat#: 97063–798 |
| CHAPS hydrate | Sigma-Aldrich | Cat#: C3023 |
| DTT | Fisher Scientific | Cat#: BP1725 |
| Protease Inhibitor Cocktail | Sigma-Aldrich | Cat#: 5892791001 |
| Polybrene | Sigma-Aldrich | Cat#: TR-1003-G |
| X-tremegene HP transfection reagent | Sigma-Aldrich | Cat#: 6366236001 |
| Cell Titer Glo reagent | Promega | Cat#: 88836 |
| KI696 | MedChemExpress | Cat#: HY-101140 |
| Bardoxolone-methyl | MedChemExpress | Cat#: HY-13324 |
| Piericidin A | Cayman | Cat#: 15379 |
| Rotenone | Sigma-Aldrich | Cat#: R8875 |
| Phenformin | Cayman | Cat#: 14997 |
| Atpenin A5 | Cayman | Cat#: 11898 |
| Antimycin A | Cayman | Cat#: 19433 |
| Sodium azide | Sigma-Aldrich | Cat#: S8032 |
| Oligomycin A | Cayman | Cat#: 11342 |
| IACS-010759 | Cayman | Cat#: 25867 |
| 2-Deoxyglucose | Sigma-Aldrich | Cat#: D8375 |
| β-Nicotinamide mononucleotide | MedChemExpress | Cat#: HY-F0004 |
| Sodium oxamate | Cayman | Cat#: 19057 |
| AZD7545 | Cayman | Cat#: 19282 |
| Phenylacetaldehyde | Sigma | Cat#: 107395 |
| 4-Hydroxyphenylacetaldehyde | This Study | N/A |
| Critical Commercial Assays | Cat#: UN3334 | |
| Seahorse XFe96 FluxPac | Agilent | Cat# 102601–100 |
| NAD/NADH-Glo™ Assay kit | Promega | Cat#: 103344–100 |
| Deposited data | ||
| RNA-seq data | This study | GEO: GSE221194 |
| Proteomics data | This study | PRIDE: PXD039027 |
| Recombinant DNA | ||
| pLenti:SoNar-NES | This study | |
| pCW57.1: FLAG-METAP2 | This study | N/A |
| pCW57.1: FLAG-LbNOX | This study | N/A |
| pCW57.1: FLAG-NDI-1 | This study | N/A |
| pCW57.1: FLAG-PFKP | This study | N/A |
| pCW57.1:FLAG-KEAP1-PAM mutant | This study | N/A |
| pLENTI-CRISPRv2:sgCTRL | This study | N/A |
| pLENTI-CRISPRv2:sgNRF2_9 | This Study | N/A |
| pLENTI-CRISPRv2:sgNRF2_10 | This Study | N/A |
| pLENTI-CRISPRv2:sgKEAP1_9 | This Study | N/A |
| pLENTI-CRISPRv2:sgKEAP1_10 | This Study | N/A |
| pLENTI-CRISPRv2:sgALDH3A1_5 | This Study | N/A |
| pLENTI-CRISPRv2:sgALDH3A1_7 | This Study | N/A |
| pLKO-Tet-On: shGFP | 111 | |
| pLKO-Tet-On: shKEAP1_1 | This Study | N/A |
| Human CRISPR Metabolic Gene Knockout Library | 28 | 110066 |
| Human Activity-Optimized CRISPR Knockout Library | 97 | 1000000100 |
| Software and Algorithms | ||
| Prism (v7.0e) | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| FlowJo (v10.0.7) | Treestar Inc. | https://www.flowjo.com/ |
| ImageJ | NIH | https://imagej.net/software/fiji/ |
| R version (4.0.3) | The R Project for Statistical Computing | https://www.r-project.org |