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. 2023 Mar 9;13:3934. doi: 10.1038/s41598-023-31141-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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CRISPR-Cas9 genetic screen identifies genes that attenuate Tun-mediated neurodegeneration. (A) Cas9 gene was inserted upstream of the endogenous GAPDH. (B) Cas9 expression was detected on d4 neurons. GAPDH and B-Actin are shown as controls. Original blots are presented in Supplementary Fig. S7. (C) Diagram showing the strategy for the screen. (D) Transduction efficiency of the three replicates. (E) Viability of Tun-treated samples (100 nM Tun for 7 days) was about 70%, as judged by quantifying the total DNA of the samples. (F) Editing efficiency of iNeurons-Cas9. (G) Gene hits ranked based on their p-value. Red line indicates p-value of 0.05. Data are shown as mean ± SEM, n = 3.