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. 2023 Mar 10;14:1307. doi: 10.1038/s41467-023-37020-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Cell viability assays of SW480 cells treated with Lipo+Uu-mRNA and nano-lantern with Uu-mRNA for 24 h, respectively. b–d The cell proliferation ability of SW480 and SW620 cells incubated with individual Smad4 mRNA or mRNA nano-lantern by (b) CCK8 assay, (c) EdU assay, Scale bar: 100 μm, and (d) plate colony formation assay. e The cell migration ability of SW480 and SW620 cells incubated with individual Smad4 mRNA or mRNA nano-lantern by a scratch wound assay. Scale bar: 100 μm. f The cell invasion of SW480 and SW620 cells was incubated with individual Smad4 mRNA or mRNA nano-lantern by in vitro transwell invasion assay. Scale bar: 50 μm. Data are presented as the mean ± SEM. n = 3 independent biological samples. Panel (a), independent-sample t-test, two-sided, statistical differences of the panel (b–f) were assessed using one-way ANOVA with Bonferroni multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.