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. 2023 Feb 24;14:1133011. doi: 10.3389/fphar.2023.1133011

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Copyright © 2023 Chen, Liu, Zhang, Huang, Zhang, Feng, Zeng, Lin, Wang and Song.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vivo as well as ex vivo biodistribution of free ICG and ICG-BSA/MnO₂ NPs following IV injection. (A) In vivo entire-animal imaging of ICG fluorescence at several time points (0.5, 1, 2, 4, 6, 8, 12, and 24 h) following IV administration of free ICG and ICG-BSA/MnO₂ NPs (the tumor zone is circled in red); (B) measuring the fluorescence intensity of tumors in MCF-7/ADR-bearing BALB/c nude mice at developed time points (0.5, 1, 2, 4, 6, 8, 12, as well as 24 h) after IV injection of free ICG along with ICG-BSA/MnO₂ NPs; (C) Ex-vivo imaging of main organs and tumors distribution of ICG following IV administration of free ICG and ICG-BSA/MnO₂ NPs for 24 h.