Table 1.
Oligonucleotide primers used in this study.
| Organism | Target gene | Primer sequence (5′-3′) | Amplification size (bp) | Annealing temperature(°C) | Reference |
|---|---|---|---|---|---|
| Dermacentor. spp | 12S rRNA | AAACTAGGATTAGATACCCT | 386 | 51 | (46) |
| AATGAGAGCGACGGGCGATGT | |||||
| 16S rRNA | ATGAAAATCTTTAAATTGCTG | 475 | 50 | (29) | |
| CCTCATTCTC ATCGGTCT | |||||
| COI | CGAATAGAACTTAGCCAACCT | 1,210 | 50 | (29) | |
| AATAACGACGGGGTATTCCT | |||||
| ITS2 | TCCGTCGACTCGTTTTGACC | 1,042 | 57 | (29) | |
| GGATAC ATCGCTTTCGCCCAT | |||||
| Rickettsia. spp | ompA | GCTTTATTCACCACCTCAAC | 209/212 | 55 | (47) |
| TRATCACCACCGTAAGTAAAT | |||||
| ompB | AAACAATAATCAAGGTACTGT | 812 | 53 | (48) | |
| TACTTCCGGTTACAGCAAAGT | |||||
| gltA | GCAAGTATCGGTGAGGATGTAAT | 401 | 48 | (49) | |
| GCTTCCTTAAAATTCAATAAATCAGGAT | |||||
| sca4 | CGATGGTAGCATTAAAAGCT | 623 | 55 | (50) | |
| CTTGCTTTTCAGCAATATCAC |