Figure 3.

Cardiac Macrophages Are Activated in HFD Hearts

(A) Monocyte chemoattractant protein (MCP)-1 level was significantly higher in HFD hearts by Western blot; n = 7 mice per group. (B) Representative flow cytometry images showing the gating strategy and analysis to identify macrophages subsets. Isolated noncardiomyocyte cells were pregated on CD11b⁺F4/80⁺ as cardiac macrophages and were further divided into subsets based on their expression of CCR2, T cell immunoglobulin and mucin domain containing 4 (Timd4), CD206, or CD86. (C) Percentage of cardiac macrophages (CD11b⁺F4/80⁺) among noncardiomyocyte interstitial cells; n = 4 to 21 mice per group. (D) Percentage change of each macrophage subset; n = 8 to 12 mice per group. Bars are mean ± SEM. Unpaired t-test (A and D) or 1-way analysis of variance with Bonferroni post hoc tests (C) were used. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001 vs Ctrl; and &P < 0.01 vs HFD. CCR2 = C-C motif chemokine receptor 2; PI = propidium iodide; other abbreviations as in Figure 1.