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. 2023 Mar 9;13:3933. doi: 10.1038/s41598-023-30922-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Sinomenine inhibited the inflammatory activity of neutrophils in vitro. (A) The effect of Sinomenine on the activity of neutrophils. CCK-8 assays were used to detect the effect of Sinomenine on neutrophil activity after four hours of culture. Statistical analysis was applied to calculate the cell survival rate of each group. The data are expressed as the mean ± SD (n = 6). (B) Western blot analysis of the levels of p-P65 and P65 in neutrophils of each group with different treatments. GAPDH was used as an internal control. Representative images are shown (working concentration of p-P65 and P65 antibodies: 1:1000). (C) The relative levels of p-P65/P65 in (B). The data are shown as the mean ± SD (n = 3). (D) Western blot analysis of the levels of p-ERK, ERK, p-P38, P38, p-JNK and JNK. GAPDH was used as an internal control. Representative images are shown (working concentration of p-ERK, ERK, p-P38, P38, p-JNK and JNK antibodies: 1:1000). (E) The relative levels of p-ERK/ERK in (D). The data are shown as the mean ± SD (n = 3). (F) The relative levels of p-P38/P38 in (D). The data are shown as the mean ± SD (n = 3). (G) The relative levels of p-JNK/JNK in (D). The data are shown as the mean ± SD (n = 3). *p < 0.05, **p < 0.01.