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. 2023 Mar 9;13:3933. doi: 10.1038/s41598-023-30922-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Sinomenine inhibited the formation of NETs in vitro. (A) IF staining was used to determine the level of NE in neutrophils. Representative images are shown. (B) The nuclear areas in (A). The data are shown as the mean ± SD. (C) The mean density of NE in (A). The data are shown as the mean ± SD (n = 3). (D) IF staining was used to determine the level of PAD4 in neutrophils in each group. Representative images are shown. (E) The nuclear areas in (D). The data are shown as the mean ± SD. (F) The mean density of PAD4 in (D). The data are shown as the mean ± SD (n = 3). (G) IF staining was used to determine the level of CitH3 in the neutrophils in each group. Representative images are shown. (H) The nuclear areas in (G). The data are shown as the mean ± SD. (I) The mean density of CitH3 in (G). The data are shown as the mean ± SD (n = 3). *p < 0.05, **p < 0.01.