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. 2023 Mar 9;14(3):187. doi: 10.1038/s41419-023-05710-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A, B An ALDEFLUOR assay was conducted in HN6 and SCC15 cells with PVT1 KD, and flow cytometry was used to quantify the percentage of ALDH^high cells. Data are shown as the mean ± SD. **p < 0.01 using an unpaired Student’s t-test. C, D qRT-PCR assessment of stemness-related marker expression in ALDH^high HN6 and SCC15 cells with PVT1 KD. Data are shown as the mean ± SD. **p < 0.01 using an unpaired Student’s t-test. E Western blot analysis of BMI1 in HN6 and SCC15 cells with PVT1 KD. F, G Image of tumorspheres formed by ALDH^high and ALDH^low HNSCC cells transfected with the indicated siRNAs. Scale bar: 100 μm. H The numbers of tumorspheres formed by ALDH^high and ALDH^low HNSCC cells transfected with the indicated siRNAs. Data are shown as the mean ± SD. **p < 0.01 using an unpaired Student’s t-test.