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. 2023 Mar 9;14(3):187. doi: 10.1038/s41419-023-05710-6

Fig. 6. Targeting PTV1 to overcome PD1 blockade resistance and to eliminate CSCs.

Fig. 6

A A schematic diagram of the experimental design for the treatment and lineage tracing of primary HNSCC in Bmi1CreER; RosatdTomato mice. To label the Bmi1+ CSCs, mice were injected with 2.5 mg Tamoxifen (Tam) in a single dose before sacrificing (Sac). B Image of tumors in tongues from the different treatment groups. The lesion areas are circled using a black dotted line. Scale bar, 2 mm. Quantification of the HNSCC lesion area of mice with different indicated treatments. Data are shown as the mean ± SD from at least two independent experiments. n = 16. *p < 0.05 and **p < 0.01 using one-way ANOVA. C Images of H&E-stained HNSCC of mice receiving different indicated treatments. Scale bar, 500 μm. Lower panels show higher magnification images. Scale bar, 100 μm. D, E Quantification of the tumor number and area of mice in the different treatment groups. Data are shown as the mean ± SD from at least two independent experiments. n = 16. *p < 0.05 and **p < 0.01 using one-way ANOVA. F Quantification of the grades of HNSCC invasion in mice from the different treatment groups. The results are presented from two independent experiments. n = 16. *p < 0.05 and **p < 0.01 using a Cochran–Armitage test. G A representative image of anti-PCK immunostaining of cervical lymph nodes. Scale bar, 200 μm. H Proportion of mice with lymph node metastasis in the different treatment groups. The number of metastatic lymph nodes is shown. Results are presented from two independent experiments. *p < 0.05 and **p < 0.01 using a χ2 test. I Quantification of lymph node metastatic areas of mice in the different treatment groups. Data are shown as the mean ± SEM from at least two independent experiments. *p < 0.05 and **p < 0.01 using one-way ANOVA. J Immunofluorescent images for CD8+ T cells from mice in the different treatment groups. The white dotted line indicates the boundary between interstitial tissue and the tumor. Scale bar, 10 μm. Percentage of immunofluorescence for CD8+ T from mice in the different treatment groups. Data are shown as the mean ± SD from at least two independent experiments. n = 16. **p < 0.01 using one-way ANOVA. K Images of mice with Tomato+ Bmi1+ CSCs in HNSCC from the different treatment groups. The white dotted line indicates the boundary between interstitial tissue and the tumor. Scale bar, 25 μm. Percentage of Tomato+ Bmi1+ CSCs in HNSCC from the different treatment groups. Data are shown as the mean ± SD from at least two independent experiments. n = 16. *p < 0.05 and **p < 0.01 using one-way ANOVA.