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. 2023 Mar 9;13:3943. doi: 10.1038/s41598-023-30565-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The BIM silencing increased lung cancer cell apoptosis compared to the PD-L1 silencing after treatment with gefitinib. (A) The western blot confirmed the silencing of PD-L1 and BIM of HCC827 cells by transfecting siRNA. (B) After transfecting with siRNA targeting PD-L1 and BIM, the HCC827 cells were cultured in control conditions (DMSO) or in the presence of the indicated doses of gefitinib, for 24 h. Flow cytometry was performed after staining cells with Annexin V-FITC and propidium iodide (PI). The cells in the Mock group were treated with transfecting vector only. (C) Quantitative analysis of Annexin V-FITC flow cytometry showed an increase in apoptotic cells in HCC827 cells with BIM silencing. *P < 0.05, **P < 0.01.