Figure 1.
StableMARK labels a subset of (acetylated) MTs. (A) StableMARK: 1–560 truncation of human Kinesin-1 containing the G234A rigor mutation fused to a tandem of mNeonGreen separated by linker sequences. (B) Cartoon: Upon expression in cells, StableMARK decorates stable MTs in a speckle-like manner. (C) Fluorescence images of U2OS cells expressing StableMARK and immunolabeled for acetylated tubulin and α-tubulin are shown in inverted contrast. (D) Zooms of the region indicated by a dashed box in C. (E) Intensity profile across the region indicated with a dashed line in D. Scale bars, 20 µm (C), 5 µm (D).