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. 2023 Mar 2;222(5):e202106105. doi: 10.1083/jcb.202106105

Figure 6.

Figure 6.

Properties of stable MTs. (A) Cartoon: Stable and dynamic MTs in the nuclear area are severed by a focused laser beam that is moved along a line. (B) Stills from live-cell imaging of U2OS cells expressing StableMARK and mCherry-tubulin. Purple marker indicates photo-ablation. Magenta arrowheads in top panels indicate examples of freshly generated, StableMARK-decorated MT-ends that remain stable upon cutting. Cyan arrowheads in the bottom panel point toward freshly generated MTs in the total tubulin channel that depolymerize upon cutting (see also Video 7). (C) Stacked bar graph representing the percentage of cut StableMARK-MTs that have stable ends vs. depolymerizing ends. Mean ± SD of in total 119 cut StableMARK-MTs from 36 cells from four independent experiments are shown. (D) Stills from live-cell imaging of U2OS cell expressing StableMARK and EB3-tdtomato (see also Video 8). (E) Kymograph showing EB3 comet growing at a StableMARK-decorated MT-tip as in D. (F) Stacked bar graph showing the percentage of StableMARK-decorated MT-ends with/without EB3 comet. Mean ± SD are shown. 97 StableMARK-decorated MT-tips from 35 cells from three independent experiments were included in this analysis. Scale bars, 2.5 µm (B and G), 1 µm (D).