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. 2023 Mar 2;222(5):e202106105. doi: 10.1083/jcb.202106105

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© 2023 Jansen et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S5. — Dynamics of StableMARK-decorated MTs. Related to Fig. 6. (A) Stills from live-cell imaging of U2OS cell expressing StableMARK (shown) and mCherry-tubulin. Purple marker indicates photo-ablation. Cyan arrowheads point toward freshly generated, StableMARK-decorated MT-end that depolymerizes upon cutting. (B) Fluorescence STED images of U2OS cell immunolabeled for acetylated tubulin and α-tubulin. (C) Zoom of region indicated with red dashed boxed in B. Magenta arrowheads indicate acetylated MT-tips that do not have dynamic ends. Cyan arrowhead points toward an acetylated MT-tip that does have a dynamic end, as manifested by a total tubulin stretch extending from the acetylated MT-tip. (D) Stacked bar graph showing the percentage of acetylated MT-ends with/without a dynamic end. 243 acetylated MT-tips from 42 cells from one independent experiment were included in this analysis. Scale bar, 5 µm (A and B), 1 µm (C). Time, s:ms.