Figure S6.

Stable MTs during cell division. Related to Fig. 8. (A) Maximum intensity projection images of U2OS WT and U2OS;StableMARK cells treated with doxycycline to induced expression of StableMARK and stained for DAPI, acetylated tubulin, and total tubulin during metaphase at 37°C or after 10 min at 4°C. (B) Maximum intensity projection images of U2OS WT cells stained for DAPI, acetylated tubulin, and total tubulin at different stages of intracellular bridge formation. Red dashed boxes indicate the regions that are analyzed for the data plotted in C. (C) Quantification showing the intensity ratio of acetylated tubulin (I_A) over total tubulin (I_T) in the regions indicated in B. Graph represents mean ± SD as well as individual values (gray circles). 23–32 regions per stage from three independent experiments were included in the analysis. Kruskal–Wallis test with Dunn’s multiple comparisons test. *P ≤ 0.05, ****P ≤ 0.0001. (D) 3D volumetric rendering of first five frames shown in Fig. 8 C. (E) Stills from live-cell imaging of U2OS;StableMARK cells treated with doxycycline to induce expression. For every time point, a Z-stack of 25 slices with 0.75 µm step size is shown as a 3D volumetric render. Magenta arrowheads indicate abscission and release of the midbody remnant. Scale bars, 5 µm (A, B, and D), 10 µm (C), 8 µm (E). Time, min:s.