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. 2023 Feb 23;220(5):e20211110. doi: 10.1084/jem.20211110

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© 2023 Seth et al.

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Figure 5. — TCR stimulation-mediated NFAT2 autoregulation is required for Tfh cell maintenance and survival. (A) Percentages and numbers of PSGL1^hiLy6c^hi, PSGL1^loLy6c^lo, and PSGL1^loLyc6^loPD1⁺CXCR5⁺ CD4⁺ T cells after day 8 LCMV infection with vehicle or CsA treatment. (B) Percentages of IFNγ⁺, IL-21⁺IFNγ⁺, and IL-21⁺ CD4⁺ T cells within each CD4⁺ T cell subset. (C) Percentages and numbers of PSGL1^hiLy6c^hi, PSGL1^loLy6c^lo, and PSGL1^loLyc6^loPD1⁺CXCR5⁺ CD4⁺ T cells after day 4 LCMV infection with vehicle or CsA treatment. (D) MFI of NFAT2 after day 4 LCMV infection in the indicated cell subsets. Data were analyzed with two-way ANOVA. (E) Percentages of PSGL1^hiLy6c^hi, PSGL1^loLy6c^lo, and PSGL1^loLyc6^loPD1⁺CXCR5⁺ CD4⁺ T cells for each indicated condition. (F) MFI of NFAT2, NFAT1, Tox or Bcl6 in PSGL1^loLyc6^loPD1⁺CXCR5⁺ CD4⁺ T cells. One-way ANOVA was used to analyze E and F. Results are representative of three independent experiments with three to five mice per experimental group. See also Fig. S4. **** = P ≤ 0.0001, *** = P ≤ 0.001, ** = P ≤ 0.01, * = P ≤ 0.05, and ns P > 0.05.