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. 2023 Feb 24;11:1090593. doi: 10.3389/fcell.2023.1090593

FIGURE 6.

FIGURE 6

ID3 mediates the BMP2-induced downregulation of ICAM1 expression in HESCs and HDSCs. (A,B) HESCs were transfected with 25 nM control siRNA (siCtrl) or 25 nM siRNA targeting ID3 (siID3) for 48 h, and the cells were then treated with vehicle control (Ctrl) or 25 ng/mL BMP2 for an additional 24 h. The mRNA and protein levels of ID3 (A) and ICAM1 (B) were examined using RT-qPCR and Western blot analysis respectively. (C,D) HDSCs were transfected with 25 nM siCtrl or 25 nM siID3 for 48 h, and the cells were then treated with Ctrl or 25 ng/ml of BMP2 for an additional 24 h. The mRNA and protein levels of ID3 (C) and ICAM1 (D) were examined using RT-qPCRand Western blot analysis, respectively. The results are expressed as the mean ± S.E.M. of at least three independent experiments. Different letters indicate significant a difference (p < 0.05).