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. 2023 Feb 24;11:1090593. doi: 10.3389/fcell.2023.1090593

FIGURE 8.

FIGURE 8

ALK3 type I mediates the BMP2-induced downregulation of ICAM1 and ID3 in HESCs and HDSCs. (A,B) HESCs were transfected with 25 nM siCtrl, siRNA targeting ALK2 (siALK2) or siRNA targeting ALK3 (siALK3) for 48 h, and the cells were then treated with Ctrl or 25 ng/mL of BMP2 for an additional 24 h. The mRNA levels of ICAM1 (A) and ID3 (B) were examined using RT-qPCR and the protein levels of ICAM1 and ID3 were examined using Western blot analysis. (C,D) HDSCs were transfected with 25 nM siCtrl, 25 nM siALK2 or 25 nM siALK3 for 48 h, and the cells were then treated with Ctrl or 25 ng/mL BMP2 for an additional 24 h. The mRNA levels of ICAM1 (C) and ID3 (D) were examined using RT-qPCR, and the protein levels of ICAM1 and ID3 were examined using Western blot analysis. The results are expressed as the mean ± S.E.M. of at least three independent experiments. Different letters indicate significant a difference (p < 0.05).