FIGURE 9.

SMAD4 is involved in the BMP2-induced upregulation of ID3 expression and downregulation of ICAM1 expression in HESCs and HDSCs. (A–C) HESCs were transfected with 25 nMsiCtrl or 25 nM siRNA targeting SMAD4 (siSMAD4) for 48 h, and the cells were then treated with BMP2 (25 ng/mL) for an additional 24 h. The mRNA and protein levels of SAMD4 (A), ICAM1 (B) and ID3 (C) were examined using RT-qPCR and Western blot analysis, respectively. (D–F) HDSCs were transfected with 25 nM siCtrl or 25 nM siSMAD4 for 48 h, and the cells were then treated with BMP2 (25 ng/mL) for an additional 24 h. The mRNA and protein levels of SAMD4 (D), ICAM1 (E) and ID3 (F) were examined using RT-qPCR and Western blot analysis. Respectively. The results are expressed as the mean ± S.E.M. of at least three independent experiments. Different letters indicate significant a difference (p < 0.05).