TABLE 2.
Plasmids used in this study.
| Plasmid | Genetic characteristics | Reference or source |
|---|---|---|
| pET-28a (PB) | An ePathBrick vector | Xu et al. (2012), Lv et al. (2017) |
| pET28a (PB)-AcceGI | pET-28a (PB) carrying a glucose isomerase gene AcceGI from Acidothermus cellulolyticus 11B | This study |
| pET28a (PB)-CcDPEase | pET-28a (PB) carrying a D-psicose 3-epimerase gene CcDPEase from Clostridium cellulolyticum H10 | This study |
| pET28a (PB)-YlXI | pET-28a (PB) carrying a putative xylose isomerase gene from Yarrowia lipolytica Po1f | This study |
| pET28a (PB)-YlGPI | pET-28a (PB) carrying a putative glucose-6-phosphate isomerase gene from Yarrowia lipolytica Po1f | This study |
| pET28a (PB)-GoDPEase | pET-28a (PB) carrying a putative D-psicose 3-epimerase or xylose isomerase( a ) gene from Gluconobacter oxydans 621H | This study |
| pET28a (PB)-GoXI_02 | pET-28a (PB) carrying a putative xylose isomerase gene from Gluconobacter oxydans 621H | This study |
| pET28a (PB)-GoGPI | pET-28a (PB) carrying a putative glucose-6-phosphate isomerase gene from Gluconobacter oxydans 621H | This study |
| pET28a (PB)-PpDPEase_01 | pET-28a (PB) carrying a putative D-psicose 3-epimerase gene from Pseudomonas putida KT2440 | This study |
| pET28a (PB)-PpDPEase_02 | pET-28a (PB) carrying a putative D-psicose 3-epimerase gene from Pseudomonas putida KT2440 | This study |
| pET28a (PB)-PpXI | pET-28a (PB) carrying a putative xylose isomerase gene from Pseudomonas putida KT2440 | This study |
| pET28a (PB)-BsDPEase_01 | pET-28a (PB) carrying a putative D-psicose 3-epimerase gene from Bacillus subtilis subsp. subtilis str. 168 | This study |
| pET28a (PB)-BsDPEase_02 | pET-28a (PB) carrying a putative D-psicose 3-epimerase gene from Bacillus subtilis subsp. subtilis str. 168 | This study |
| pET28a (PB)-BsXI | pET-28a (PB) carrying a putative xylose isomerase gene from Bacillus subtilis subsp. subtilis str. 168 | This study |
| pET28a (PB)-BsGPI | pET-28a (PB) carrying a putative glucose-6-phosphate isomerase gene from Bacillus subtilis subsp. subtilis str. 168 | This study |
| pET28a (PB)-PaXI | pET-28a (PB) carrying a putative xylose isomerase gene from Pseudomonas aeruginosa PAO1 | This study |
| pET28a (PB)-PaDPEase_01 | pET-28a (PB) carrying a putative D-psicose 3-epimerase gene from Pseudomonas aeruginosa PAO1 | This study |
| pET28a (PB)-PaDPEase_02 | pET-28a (PB) carrying a putative D-psicose 3-epimerase gene from Pseudomonas aeruginosa PAO1 | This study |
| pET28a (PB)-BtDPEase | pET-28a (PB) carrying a putative D-psicose 3-epimerase or xylose isomerase( a ) gene from Bacillus thuringiensis ATCC 10792 | This study |
| pET28a (PB)-GP | pET-28a (PB) carrying AcceGI and CcDPEase in fusion form | This study |
| pET28a (PB)-GSP | pET-28a (PB) carrying AcceGI and CcDPEase linked with “GGGGS” encoding sequence | This study |
| pET28a (PB)-GS2P | pET-28a (PB) carrying AcceGI and CcDPEase linked with “GGGGSGGGGS” encoding sequence | This study |
| pET28a (PB)-GS3P | pET-28a (PB) carrying AcceGI and CcDPEase linked with “GGGGSGGGGSGGGGS” encoding sequence | This study |
| pET28a (PB)-GEP | pET-28a (PB) carrying AcceGI and CcDPEase linked with “EAAAK” encoding sequence | This study |
| pET28a (PB)-GE2P | pET-28a (PB) carrying AcceGI and CcDPEase linked with “EAAAKEAAAK” encoding sequence | This study |
| pET28a (PB)-GE3P | pET-28a (PB) carrying AcceGI and CcDPEase linked with “EAAAKEAAAKEAAAK” encoding sequence | This study |
| pET28a (PB)-AcceGI-CcDPEase | pET-28a (PB) carrying AcceGI and CcDPEase in monocistronic form | This study |
| pET28a (PB)-AcceGI×2-CcDPEase | pET-28a (PB) carrying 2-copy AcceGI and CcDPEase in monocistronic form | This study |
| pET28a (PB)-AcceGI-CcDPEase×2 | pET-28a (PB) carrying AcceGI and 2-copy CcDPEase in monocistronic form | This study |
| pET28a (PB)-AcceGI×3-CcDPEase | pET-28a (PB) carrying 3-copy AcceGI and CcDPEase in monocistronic form | This study |
| pET28a (PB)-AcceGI×4-CcDPEase | pET-28a (PB) carrying 4-copy AcceGI and CcDPEase in monocistronic form | This study |
| pET28a (PB)-AcceGI×5-CcDPEase | pET-28a (PB) carrying 5-copy AcceGI and CcDPEase in monocistronic form | This study |
| pET28a (PB)-AcceGI×6-CcDPEase | pET-28a (PB) carrying 6-copy AcceGI and CcDPEase in monocistronic form | This study |
| pET28a (PB)-AcceGI×7-CcDPEase | pET-28a (PB) carrying 7-copy AcceGI and CcDPEase in monocistronic form | This study |
a
These genes were annotated as both D-psicose 3-epimerase and xylose isomerase in the local blast analysis.