FIGURE 6.

Expression changes of TPH2 in (A) the dorsal raphe nucleus (DRN) and (B) caudal raphe nucleus (CRN) of brainstem. Light intensity programs were changed at day 7–5, 20, 480 lx (NL house), 2–5 lx/40 lx (VL house). Brains of male bird were sampled on days 14, 28 and 42 (n = 12/section, 4 sections/house). DRN and CRN of the brainstem from each bird were dissected as described in Figure 5. Total RNA was extracted and used for real time RT-qPCR for TPH2 expression. Data were set as the relative fold changes of expression levels using the ΔΔCt method with GAPDH and β-actin as internal controls. Data (mean ± SEM) were expressed from a value set for 1.0 for 5 lx birds at 14 days of age. Different lower-case letters above the bars denote significant differences (p < 0.05) among groups, where a>b>c>d>e>f>g and a,b is not different from a or b.