TABLE 1.

Primers used for RT-qPCR.

Gene	GenBank #	Primer sequence (5′–3′)	Size (bp)	Annealing Tm (°C)
TPH2	NM_001001301.1	F: AGG​ACC​TCC​GCA​GTG​ATC​TA	111	58
		R: CAG​CAT​AAG​CAG​CTG​ACA​ACA
TH	NM_204805	F: CTT​TGA​TCC​TGA​TGC​TGC​TG	103	56
		R: CCT​CAG​CTT​GTT​TTT​GGC​AT
GR	NM_001037826	F: GCC​ATC​GTG​AAA​AGA​GAA​GG	95	54
		R: TTT​CAA​CCA​CAT​CGT​GCA​T
BDNF	NM_001031616	F: GAC​ATG​GCA​GCT​TGG​CTT​AC	167	60
		R: GTT​TTC​CTC​ACT​GGG​CTG​GA
Opn4	AY036061	F: AAG​GTT​TCG​CTG​TCA​TCC​AGC	128	58
		R: CTG​CTG​CTG​TTC​AAA​CCA​AC
GAPDH	NM 204305	F: CTT​TGG​CAT​TGT​GGA​GGG​TC	128	58–60
		R: ACG​CTG​GGA​TGA​TGT​TCG​G
$\mathrm{\beta }$ -actin	L08165	F: CAC​AAT​GTA​CCC​TGG​CAT​TG	158	54–56
		R: ACA​TCT​GCT​GGA​AGG​TGG​AC