Figure S5.

Functional impact of iCD4+ T cells in the context of HIV infection and anti-tumor response. (A) Representative cotransduction rates of control (GFP) or RELA K5R and CAR (CD19-Fc staining) lentivectors in T cells. (B) Rates of CAR+ cells in control (GFP) or RELA K5R transduced T cells (n = 10 donors combined from five independent experiments). Each symbol represents one donor, bar indicates mean, paired one-way ANOVA with Tukey’s multiple comparison test. (C) Bar plots representation of data shown in Fig. 7 C on days 0, 2, and 5 after coculture of CAR+ GFP+ or CAR+ RELA K5R+ T cells with mKate2+CD19⁺ A549 cells (n = 10 donors combined from five independent experiments). (D) IFNλ1 concentration at the end of the coculture period with 1,000, 500, and 250 CAR+ GFP+ or CAR+ RELA K5R+ T cells (n = 6 donors combined from three independent experiments). (E) IFN-I/III concentration 48 h following HIV-1 or HIV-2 infection of CD4⁺ T cells transduced with the indicated lentivectors and pretreated with 5AZA (2 µM) for 48 h (n = 4 donors combined from two independent experiments). Each symbol represents one donor, bar represents geometric mean, paired one-way ANOVA with Tukey’s multiple comparison test, *P ≤ 0.05, **P ≤0.01, ***P ≤ 0.001, ****P ≤ 0.0001, ns, not significant.