Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 23;220(5):e20221563. doi: 10.1084/jem.20221563

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Wei et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — CRISPR screens for SHP099 resistance. (A) Workflow for genome-wide CRISPR KO screens. (B and C) Results of genome-wide CRISPR KO screens of MOLM13 (B) and MV4-11 (C) cells, analyzed by MaGeCK; red circles indicate enriched genes with FDR < 0.05, blue circles label enriched genes with 0.05 < FDR < 0.1, and green circles show genes with 0.1 < FDR < 0.2. (D) Workflow for focused CRISPR mini-screens of 14 cancer lines. (E and F) Results of CRISPR mini-screens of MOLM13 (E) and MV4-11 (F) cells. Points represent log₂-fold enrichment (SHP099 vs. DMSO) of each sgRNA present in the library targeting the indicated gene compared with non-targeting (ctrl) sgRNAs; significance was assessed by Student’s t test with FDR correction. (G) Heat map showing significant hits in CRISPR mini-screens across 14 cancer lines. Yellow color indicates FDR < 0.05 enrichment for a given gene in a cell line.