TABLE 1.
Summary of properties of 5S rRNA mutants
| Sequence typeb | No. of alleles (%) conferring indicated phenotypec
|
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Growtha
|
Nonsense suppression
|
Killer (229)
|
||||||||||||
| Lethal (246 alleles) | 13°C (239)
|
37°C (236)
|
ade2-1 (239)
|
can1-100 (223)
|
||||||||||
| WT | cs− | WT | ts− | WT | Hyperacc. | Supps | WT | Suppw | Supps | WT | Kw | K− | ||
| Consensus | 2 | 118 (92) | 1 (1) | 90 (69) | 2 (1) | 94 (73) | 2 (1) | 34 (26) | 85 (70) | 1 (1) | 36 (29) | 72 (58) | 21 (16) | 33 (26) |
| Noncons. | 2 | 107 (98) | 0 | 88 (84) | 2 (2) | 94 (86) | 3 (3) | 12 (11) | 90 (88) | 1 (1) | 10 (11) | 76 (73) | 12 (12) | 15 (15) |
| Total | 4 | 225 (94) | 1 (<1) | 178 (75) | 4 (2) | 188 (79) | 5 (2) | 46 (19) | 173 (78) | 2 (1) | 47 (21) | 148 (65) | 33 (14) | 48 (21) |
a
General growth phenotypes.
b
Consensus and noncons., consensus and nonconsensus eukaryotic 5S rRNA sequences, respectively, of mutant alleles.
c
Total numbers of alleles tested are in parentheses next to phenotypes and allele designations. WT, wild type; Hyperacc., hyperaccurate; Supps, strong suppression; Suppw, weak suppression.