Antinociception effect of alcohol was measured by the hot plate test expressed as %MPE (Maximum possible effect), at different time points 15, 30, 60, 90 and 120 min after alcohol gavage was measured in (A) B6 male mice, (B) D2 male mice, (C) B6 female mice and (D) D2 female mice. (E) Comparison of the AUC of time-course of antinociceptive effect of alcohol measured by hot plate in all mice (Results were compared using two-way ANOVA with sex and strain as a factor and post-hoc Sidak’s test). Values are expressed as mean ± SEM. n= 8/group- (*p<0.05; **p<0.01 and ***p<0.001 vs. vehicle).