(A) Visual depiction of the experimental design for repeated alcohol exposure. Antinociception effect of alcohol after repeated oral administration, measured by the hot plate test as expressed by %MPE (Maximum possible effect), at different maintenance doses, 0.5, 1 and 2 g/kg, 30 min after alcohol gavage in (B) B6 male mice and (C) D2 male mice. Values are expressed as mean ± SEM. n= 8/group. Results were compared using a one-way ANOVA, followed by a post-hoc Dunnett’s test (*: p<0.05, **: p<0.01 and ***: p<0.001 vs. control) (#: p<0.05, ##: p<0.01 and ###: p<0.001 vs. vehicle). (D) The percent of tolerance measured by dose in B6 and D2 male mice.