Robustness and ruggedness study of the proposed UPLC-MS/MS method for determination of DUV in human plasma samples.
| Parameters | Recoverya (% ± RSD) |
|---|---|
| Robustness | |
| Ratio of acetonitrile : ammonium formate (10 mM) | |
| 40 : 60 | 98.44 ± 1.17 |
| 45 : 55 | 97.60 ± 1.38 |
| pH | |
| 4 | 100.49 ± 2.20 |
| 4.1 | 101.96 ± 1.26 |
| Ruggedness | |
| Instrument-to-instrument | |
| Instrument-1 | 99.56 ± 1.18 |
| Instrument-2 | 98.94 ± 3.05 |
| Analyst-to-analyst | |
| Analyst-1 | 99.83 ± 3.47 |
| Analyst-2 | 102.00 ± 4.02 |
| Day-to-day | |
| Day-1 | 99.76 ± 2.38 |
| Day-2 | 98.74 ± 3.85 |
a
Values are mean of 3 determinations.