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. 2023 Feb 23;145(9):5083–5091. doi: 10.1021/jacs.2c10838

Figure 2.

Figure 2

Selection of triterpenoid biosynthesis gene candidates by self-organizing map (SOM) analysis. Candidate genes co-expressed with the two pathway genes AaCYP71CD4 and AaCYP71BQ17 were obtained by self-organizing map analysis from multidimensional RNA-Seq expression data of A. altissima transcripts. Both CYP genes were grouped in the same cluster with the highest quality that contained 695 transcripts. The 695 contigs were manually filtered (Figure S2), leading to 20 oxidoreductase and 3 isomerase gene candidates, which were functionally evaluated by transient expression in N. benthamiana. See the main text and Supporting Information for further details. The figure was created with BioRender.com.