FIG. 1.

Schematic representation of Lyn constructs. All constructs were tagged at the C terminus with enhanced GFP (solid circle). LynA-WT and LynB-WT are constructs with two forms of wild-type Lyn kinase that differ in a 20-amino-acid-long insert in the unique domain (shaded box). LynB-CA, LynB-GA, and LynB-GCA are constructs mutated in the palmitoylation, myristoylation, or both acylation sites, respectively. LynA-UNI and LynB-UNI contain only the N-terminal unique domains of LynA and LynB, respectively. LynB-CI is catalytically inactive Lyn B (mutation of Lys279 to Arg). LynA-SH2 is LynA without the catalytic domain. Arrows mark sites of introduced point mutations. The kinase activity of all Lyn constructs is indicated as full (+) and null (−) kinase activity.