LOCUS vCpxR 3670 bp DNA circular 04-JAN-2022 DEFINITION . ACCESSION VERSION KEYWORDS . SOURCE . ORGANISM . . COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers CDS 707..1419 /note="pLannotate" /database="addgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="CDS" /locus_tag="GFP" /label="GFP" /ApEinfo_label="GFP" /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS complement(2147..2805) /note="pLannotate" /database="addgene" /identity="99.545" /match_length="100.0" /fragment="False" /other="CDS" /locus_tag="CmR" /label="CmR" /ApEinfo_label="CmR" /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin complement(join(3435..3670,1..309)) /note="pLannotate" /database="addgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="rep_origin" /locus_tag="p15A ori" /label="p15A ori" /ApEinfo_label="p15A ori" /ApEinfo_fwdcolor="#999999" /ApEinfo_revcolor="#999999" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter complement(2807..2908) /note="pLannotate" /database="addgene" /identity="99.029" /match_length="100.0" /fragment="False" /other="promoter" /locus_tag="cat promoter" /label="cat promoter" /ApEinfo_label="cat promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator complement(1485..1578) /note="pLannotate" /database="addgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="terminator" /locus_tag="lambda t0 terminator" /label="lambda t0 terminator" /ApEinfo_label="lambda t0 terminator" /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" terminator 1922..1986 /note="pLannotate" /database="addgene" /identity="100.0" /match_length="91.66666666666666" /fragment="True" /other="terminator" /locus_tag="rrnB T1 terminator" /label="rrnB T1 terminator" /ApEinfo_label="rrnB T1 terminator" /ApEinfo_fwdcolor="#9d1b1c" /ApEinfo_revcolor="#9d1b1c" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 1634..1662 /note="pLannotate" /database="addgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="CDS" /locus_tag="Myc" /label="Myc" /ApEinfo_label="Myc" /ApEinfo_fwdcolor="#e9d024" /ApEinfo_revcolor="#e9d024" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" mobile_element complement(3030..3172) /note="pLannotate" /database="addgene" /identity="100.0" /match_length="18.75" /fragment="True" /other="mobile element" /locus_tag="insertion sequence:IS1" /label="insertion sequence:IS1" /ApEinfo_label="insertion sequence:IS1" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 1594..1613 /note="pLannotate" /database="addgene" /identity="100.0" /match_length="20.588235294117645" /fragment="True" /other="promoter" /locus_tag="cat promoter(1)" /label="cat promoter(1)" /ApEinfo_label="cat promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin complement(3292..3346) /note="pLannotate" /database="addgene" /identity="100.0" /match_length="7.466666666666668" /fragment="True" /other="rep_origin" /locus_tag="RSF ori" /label="RSF ori" /ApEinfo_label="RSF ori" /ApEinfo_fwdcolor="#999999" /ApEinfo_revcolor="#999999" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ncRNA 1803..1917 /note="pLannotate" /database="infernal" /identity="100.0" /match_length="97.47899159663865" /fragment="False" /other="ncRNA" /locus_tag="5S rRNA" /label="5S rRNA" /ApEinfo_label="5S rRNA" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 402..670 /locus_tag="pCpxP promoter" /label="pCpxP promoter" /ApEinfo_label="pCpxP promoter" /ApEinfo_fwdcolor="#346ee0" /ApEinfo_revcolor="#346ee0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag 61 tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga 121 ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga 181 ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga 241 aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa 301 acgatctcaa gaagatcatc ttattaatca gataaaatat ttctagattt cagtgcaatt 361 tatctcttca aatgtagcac ctgaagtcgg atcgcggccg ctaataggga agtcagctct 421 cggtcatcat caactaacag gattttattc attgtttaaa tacctccgag gcagaaatta 481 cgtcatcaga cgtcgctaat ccatgacttt acgttgtttt acaccccctg acgcatgttt 541 gcagcctgaa tcgtaaactc tctatcgttg aatcgcgaca gaaagatttt gggagcaaat 601 gatgcgcata gttaccgctg ccgtcatggc ctcaacgctg gcagtcagtt cattaagcca 661 cgctgctgaa ggatcctctg tttattttca ggagtcatca ttatttatga gtaaaggaga 721 agaacttttc actggagttg tcccaattct tgttgaatta gatggtaatg ttaatgggca 781 caaattttct gtcagtggag agggtgaagg tgatgcagca tacggaaaac ttacccttaa 841 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttgac 901 ttatggtgtt caatgctttt caagataccc agatcatatg aaacggcatg actttttcaa 961 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa 1021 ctataagaca cgtgctgaag tcaagtttga aggtgataca cttgttaata gaatcgagtt 1081 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaagttgg aatacaacta 1141 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt 1201 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa 1261 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc 1321 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac 1381 agctgctggg attacacatg gcatggatga actatacaaa aggcctgcag caaacgacga 1441 aaactacgct taagtagctt aataagctta attagctgag cttggactcc tgttgataga 1501 tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc 1561 gttttttatt ggtgagaatc caagctagct tggcgagatt ttcaggagct aagggaattc 1621 gaagcttggg cccgaacaaa aactcatctc agaagaggat ctgaatagcg ccgtcgacca 1681 tcatcatcat catcattgag tttaaacggt ctccagcttg gctgttttgg cggatgagag 1741 aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat 1801 ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa 1861 cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca 1921 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 1981 ggtgaactaa ttcgaagctt gacataagcg gctatttaac gaccctgccc tgaaccgacg 2041 accgggtcga atttgctttc gaatttctgc cattcatccg cttattatca cttattcagg 2101 cgtagcaacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct 2161 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac 2221 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat 2281 atttgcccat cgtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa 2341 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt 2401 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa 2461 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat 2521 gtaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg 2581 ccatacggaa ctccggatga gcattcatca ggcgggcaag aatgtggata aaggccggat 2641 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg 2701 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc 2761 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag 2821 ctcctgaaaa tctcgataac tcaaaaaata tgcccggtag tgatcttatt tcattatggt 2881 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg 2941 gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca 3001 caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg atttagtgta 3061 tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca 3121 gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctagcggagt 3181 gtatactggc ttactatgtt ggcactgatg agagtgtcag tgaagtgctt catgtggcag 3241 gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc 3301 ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga 3361 acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc 3421 gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc 3481 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc 3541 ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta 3601 tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa 3661 gctggactgt //