TableS1
library name,title,organism,cell type,tissue,experimental condition,Model ,Alleles,Treatment,Sorting strategy,Time point (analyses),Sex,molecule,single or paired-end,instrument model,description
DACD511_Kate_plus,Epithelial_N1_1,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,N1,C;RIK,p48Cre;RIK,PBS / Day 2,mKate2+;Cd45-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD550_kate_plus,Epithelial_N1_2,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,N1,C;RIK,p48Cre;RIK,PBS / Day 2,mKate2+;Cd45-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DAC_B530-Kate+,Epithelial_N2_1,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,N2 ,C;RIK,p48Cre;RIK,Caerulein / Day 2,mKate2+;Cd45-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina HiSeq 2500,"10x Genomics (3' kit, v2.0)"
DACD403_Kate_plus,Epithelial_N2_2,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,N2 ,C;RIK,p48Cre;RIK,Caerulein / Day 2,mKate2+;Cd45-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD394_Kate_plus,Epithelial_K1_1,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K1,KC;RIK-shRen.713 (Off Dox),p48Cre;RIK;LSL-KrasG12D,PBS / Day 2,mKate2+;Cd45-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD406_Kate_plus,Epithelial_K1_2,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K1,KC;RIK,p48Cre;RIK;LSL-KrasG12D,PBS / Day 2,mKate2+;Cd45-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD351-Kate+,Epithelial_K1_3,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K1,KC;RIK-shRNA (Off Dox),p48Cre;RIK;LSL-KrasG12D,PBS / Day 2,mKate2+;Cd45-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DAC_C263_EPI,Epithelial_K1.5_1,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K1.5,KC;RIK-shRNA (Off Dox),p48Cre;RIK;LSL-KrasG12D,PBS / Week 3,mKate2+;Cd45-;DAPI-,8-9 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
D396_EPI,Epithelial_K1.5_2,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K1.5,KC;RIK-shRNA (Off Dox),p48Cre;RIK;LSL-KrasG12D,PBS / Week 3,mKate2+;Cd45-;DAPI-,8-9 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD404_Kate_plus,Epithelial_K2_1,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K2,KC;RIK,p48Cre;RIK;LSL-KrasG12D,Caerulein / Day 2,mKate2+;Cd45-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD407_Kate_plus,Epithelial_K2-Day1_1,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K2-Day1,KC;RIK,p48Cre;RIK;LSL-KrasG12D,Caerulein / Day 1,mKate2+;Cd45-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DAC_DI143_Epi,Epithelial_K3_1,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K3,KC;RIK,p48Cre;RIK;LSL-KrasG12D,Caerulein / Week 3,mKate2+;Cd45-;DAPI-,8-9 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD482_Kate_plus,Epithelial_K3_2,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K3,KC;RIK,p48Cre;RIK;LSL-KrasG12D,Caerulein / Week 3,mKate2+;Cd45-;DAPI-,8-9 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DAC_C301-EPI_1,Epithelial_K4_1,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K4,KC;RIK,p48Cre;RIK;LSL-KrasG12D,n/a,mKate2+;Cd45-;DAPI-,27 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DAC_C301-EPI_2,Epithelial_K4_2,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K4,KC;RIK,p48Cre;RIK;LSL-KrasG12D,n/a,mKate2+;Cd45-;DAPI-,27 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
Ag-PDAC-PT-Kate,Epithelial_K5_1,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K5,KPC;RIK,p48Cre;RIK;LSL-KrasG12D;p53R172H,PDAC lesion (Primary Tumor),mKate2+;Cd45-;DAPI-,PDAC lesion,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
Ag-Lung-Mets-Kate,Epithelial_K6_1,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K6,KPC;RIK,p48Cre;RIK;LSL-KrasG12D;p53R172H,PDAC lesion (Lung Metastases),mKate2+;Cd45-;DAPI-,PDAC lesion,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DAC_D020_p5_Epi,Epithelial_K5_3,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K5,KPC;RIK,p48Cre;RIK;LSL-KrasG12D;p53fl/wt,PDAC lesion (Primary Tumor),mKate2+;Cd45-;DAPI-,PDAC lesion,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACC963PT_Kate_plus,Epithelial_K5_4,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K5,KPC;RIK,p48Cre;RIK;LSL-KrasG12D;p53fl/wt,PDAC lesion (Primary Tumor),mKate2+;Cd45-;DAPI-,PDAC lesion,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACC963LIVERmet,Epithelial_K6_4.1,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K6,KPC;RIK,p48Cre;RIK;LSL-KrasG12D;p53fl/wt,PDAC lesion (Liver Metastases),mKate2+;Cd45-;DAPI-,PDAC lesion,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACC963_mKate_plus,Epithelial_K6_4.2,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K6,KPC;RIK,p48Cre;RIK;LSL-KrasG12D;p53fl/wt,PDAC lesion (Lung Metastases),mKate2+;Cd45-;DAPI-,PDAC lesion,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD350-Kate+,Epithelial_K2shControl_1,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K2-shControl,KC;RIK-shIl33.668 (Off Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Il33 (sh668)",Caerulein / Day 2,mKate2+;Cd45-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACE621-mKate2,Epithelial_K2shControl_2,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K2-shControl,KC;RIK-shRen.713 (On Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Renilla (sh713)",Caerulein / Day 2,mKate2+;Cd45-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD346-Kate+,Epithelial_K2shIL33KD_1,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K2-shIl33-#1,KC;RIK-shIl33.668 (On Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Il33 (sh668)",Caerulein / Day 2,mKate2+;Cd45-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACE604-mKate2,Epithelial_K2shIL33KD_2,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K2-shIl33-#1,KC;RIK-shIl33.668 (On Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Il33 (sh668)",Caerulein / Day 2,mKate2+;Cd45-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACE605-mKate2,Epithelial_K2shIL33KD_3,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K2-shIl33-#1,KC;RIK-shIl33.668 (On Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Il33 (sh668)",Caerulein / Day 2,mKate2+;Cd45-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD349_mKate2+;GFP+,Epithelial_K3shControl_1,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K3-shControl,KC;RIK-shIl33.668 (Off Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Il33 (sh668)",Caerulein / Week 3,mKate2+;Cd45-;DAPI-,8-9 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACE610-EPI,Epithelial_K3shControl_2,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K3-shControl,KC;RIK-shIl33.327 (Off Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Il33 (sh327)",Caerulein / Week 3,mKate2+;Cd45-;DAPI-,8-9 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD347_mKate2+;GFP+,Epithelial_K3shIL33KD_1,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K3-shIl33-#1,KC;RIK-shIl33.668 (On Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Il33 (sh668)",Caerulein / Week 3,mKate2+;Cd45-;DAPI-,8-9 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACE607-EPI,Epithelial_K3shIL33KD_2,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K3-shIl33-#1,KC;RIK-shIl33.668 (On Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Il33 (sh668)",Caerulein / Week 3,mKate2+;Cd45-;DAPI-,8-9 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACE614-EPI,Epithelial_K3shIL33KD_3,Mus musculus,sorted primary pancreatic epithelial cells (mKate2+),pancreas,K3-shIl33-#2,KC;RIK-shIl33.327 (OnDox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Il33 (sh327)",Caerulein / Week 3,mKate2+;Cd45-;DAPI-,8-9 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD407_CD45+_Day1,Immune_K2-Day1_1,Mus musculus,sorted primary CD45+ immune cells,pancreas,K2-Day1,KC;RIK,p48Cre;RIK;LSL-KrasG12D,Caerulein / Day 1,CD45+;mKate2-;DAPI-,5 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD404_CD45,Immune_K2_1,Mus musculus,sorted primary CD45+ immune cells,pancreas,K2,KC;RIK,p48Cre;RIK;LSL-KrasG12D,Caerulein / Day 2,CD45+;mKate2-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD143_CD45+,Immune_K3_1,Mus musculus,sorted primary CD45+ immune cells,pancreas,K3,KC;RIK,p48Cre;RIK;LSL-KrasG12D,Caerulein / Week 3,CD45+;mKate2-;DAPI-,8-9 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD482_CD45+,Immune_K3_2,Mus musculus,sorted primary CD45+ immune cells,pancreas,K3,KC;RIK,p48Cre;RIK;LSL-KrasG12D,Caerulein / Week 3,CD45+;mKate2-;DAPI-,8-9 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD406-CD45+,Immune_K1_1,Mus musculus,sorted primary CD45+ immune cells,pancreas,K1,KC;RIK,p48Cre;RIK;LSL-KrasG12D,PBS / Day 2,CD45+;mKate2-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD408-CD45+,Immune_K1_2,Mus musculus,sorted primary CD45+ immune cells,pancreas,K1,KC;RIK,p48Cre;RIK;LSL-KrasG12D,PBS / Day 2,CD45+;mKate2-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD351-CD45+,Immune_K1_3,Mus musculus,sorted primary CD45+ immune cells,pancreas,K1,KC;RIK-shIl33.668 (Off Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Il33 (sh668)",PBS / Day 2,CD45+;mKate2-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD350-CD45+,Immune_K2shControl_1,Mus musculus,sorted primary CD45+ immune cells,pancreas,K2-shControl,KC;RIK-shIl33.668 (Off Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Il33 (sh668)",Caerulein / Day 2,CD45+;mKate2-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACE621-CD45,Immune_K2shControl_2,Mus musculus,sorted primary CD45+ immune cells,pancreas,K2-shControl,KC;RIK-shRen.713 (On Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Renilla (sh713)",Caerulein / Day 2,CD45+;mKate2-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD346-CD45+,Immune_K2shIL33KD_1,Mus musculus,sorted primary CD45+ immune cells,pancreas,K2-shIl33-#1,KC;RIK-shIl33.668 (On Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Il33 (sh668)",Caerulein / Day 2,CD45+;mKate2-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACE604-CD45,Immune_K2shIL33KD_2,Mus musculus,sorted primary CD45+ immune cells,pancreas,K2-shIl33-#1,KC;RIK-shIl33.668 (On Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Il33 (sh668)",Caerulein / Day 2,CD45+;mKate2-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACE605-CD45,Immune_K2shIL33KD_3,Mus musculus,sorted primary CD45+ immune cells,pancreas,K2-shIl33-#1,KC;RIK-shIl33.668 (On Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Il33 (sh668)",Caerulein / Day 2,CD45+;mKate2-;DAPI-,5-6 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD349_CD45+,Immune_K3shControl_1,Mus musculus,sorted primary CD45+ immune cells,pancreas,K2-shControl,KC;RIK-shIl33.668 (Off Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Il33 (sh668)",Caerulein / Week 3,CD45+;mKate2-;DAPI-,8-9 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACE610-CD45,Immune_K3shControl_2,Mus musculus,sorted primary CD45+ immune cells,pancreas,K2-shControl,KC;RIK-shIl33.668 (Off Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Il33 (sh668)",Caerulein / Week 3,CD45+;mKate2-;DAPI-,8-9 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD345_CD45+,Immune_K3shIL33KD_1,Mus musculus,sorted primary CD45+ immune cells,pancreas,K2-shIl33-#1,KC;RIK-shIl33.668 (On Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Il33 (sh668)",Caerulein / Week 3,CD45+;mKate2-;DAPI-,8-9 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACD347_CD45+,Immune_K3shIL33KD_2,Mus musculus,sorted primary CD45+ immune cells,pancreas,K2-shIl33-#1,KC;RIK-shIl33.668 (On Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Il33 (sh668)",Caerulein / Week 3,CD45+;mKate2-;DAPI-,8-9 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACE607-CD45,Immune_K3shIL33KD_3,Mus musculus,sorted primary CD45+ immune cells,pancreas,K2-shIl33-#1,KC;RIK-shIl33.668 (On Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Il33 (sh668)",Caerulein / Week 3,CD45+;mKate2-;DAPI-,8-9 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"
DACE614-CD45,Immune_K3shIL33KD_4,Mus musculus,sorted primary CD45+ immune cells,pancreas,K2-shIl33-#2,KC;RIK-shIl33.327 (On Dox),"KC-GEMM-ESC derived from p48-9C ESCs (Saborowski et al., PNAS, 2013) targeted with the TRE-GFP-shRNA against Il33 (sh327)",Caerulein / Week 3,CD45+;mKate2-;DAPI-,8-9 weeks old,Male,total RNA,paired-end,Illumina NovaSeq 6000,"10x Genomics (3' kit, v2.0)"